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Abstract Title:

Xanthohumol Inhibits TGF-β1-Induced Cardiac Fibroblasts Activation via Mediating PTEN/Akt/mTOR Signaling Pathway.

Abstract Source:

Drug Des Devel Ther. 2020 ;14:5431-5439. Epub 2020 Dec 7. PMID: 33324040

Abstract Author(s):

Chuanhao Jiang, Ning Xie, Taoli Sun, Wanjun Ma, Bikui Zhang, Wenqun Li

Article Affiliation:

Chuanhao Jiang

Abstract:

Background: Xanthohumol (Xn) is the most abundant prenylated flavonoid in Hops (L.), and exhibits a range of pharmacological activities. This study aimed to investigate the effect of Xn on TGF-β1-induced cardiac fibroblasts activation and elucidate the underlying mechanism.

Materials and Methods: The cellTiter 96AQueous one solution cell proliferation assay kit was adopted to determine the cell viability of cardiac fibroblasts, and the proliferation was detected through 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay. Theα-SMA protein expression was measured by using immunofluorescence and Western blotting. Western blotting was conducted to test the protein expressions of collagen I and III, PTEN, p-Akt, Akt, p-mTOR, mTOR, p-Smad3, Smad3 and GAPDH. The mRNA levels of α-SMA, collagen I and III were determined by quantitative real-time polymerase chain reaction (PCR).

Results: Xn inhibited the TGF-β1-induced proliferation, differentiation and collagen overproduction of cardiac fibroblasts. TGF-β1 induced the down-regulated PTEN expression, Akt and mTOR phosphorylation. These effects of TGF-β1 were suppressed by Xn, while blocking of PTEN reduced Xn-mediated inhibitory effect on cardiac fibroblasts activation induced by TGF-β1.

Conclusion: Xn inhibits TGF-β1-induced cardiac fibroblasts activation via mediating PTEN/Akt/mTOR signaling pathway.

Study Type : In Vitro Study

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Sayer Ji
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