Yellow lupin polysaccharides: Antioxidant, immunomodulatory and prebiotic activities and their structural characterisation. - GreenMedInfo Summary

Yellow lupin polysaccharides (YLP-1, YLP-2 and YLP-3) were isolated from the whole seeds of Lupinus luteus L. Their antioxidant activities were evaluated by ABTSand hydroxyl radical scavenging, and Fechelating assays. Immunostimulatory activities were measured by their ability to activate macrophages to produce TNF-α and NO. Four strains of probiotic bacteria were used to measure their prebiotic activities. YLP-2 with largest galactose content displayed the best activity amongst the three isolated polysaccharides. NMR and FT-IR spectroscopic methods have revealed that YLPs contain galactans and galactomannanswhich are linked with β-(1,4) glycosidic bond in the main chain. The side chain Galp unit of galactomannan is connected to the main chain Manp by α-(1,6) linkage. The results presented in this paper strongly suggest that YLPs display significant antioxidant, immunostimulatory and prebiotic activities and hence hold great potential as nutraceutical and functional agents.