12-lipoxygenase (12-LOX) https://greenmedinfo.com/category/pharmacological-actions/12-lipoxygenase-12-lox en An in-vitro test of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) inhibition potency verified that both Plantago lanceolata and P. major exhibit considerable anti-inflammatory activity. https://greenmedinfo.com/article/vitro-test-cyclooxygenase-1-cox-1-and-12-lipoxygenase-12-lox-inhibition-potenc PMID:  J Pharm Biomed Anal. 2010 Sep 5 ;52(5):701-6. Epub 2010 Feb 18. PMID: 20219312 Abstract Title:  Liquid chromatography/tandem mass spectrometry study of anti-inflammatory activity of plantain (Plantago L.) species. Abstract:  To evaluate anti-inflammatory activity of selected Plantago species (P. lanceolata L. and P. major L.) an optimized in vitro test for determination of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) inhibition potency was undertaken. By using intact cell system (platelets) as a source of COX-1 and 12-LOX enzymes and highly sensitive and specific LC-MS/MS technique for detection of main arachidonic acid metabolites formed by COX-1 and 12-LOX, this test provides efficient method for evaluation of anti-inflammatory potential of plant extracts and isolated compounds. Our results validated the well-known COX-1 inhibitory activity of P. lanceolata and P. major methanol extracts (concentration required for 50% inhibition (IC(50)) was 2.00 and 0.65 mg/ml, respectively). Furthermore, 12-LOX inhibitory activity of examined extracts was reported for the first time (IC(50)=0.75 and 1.73 mg/ml for P. lanceolata and P. major, respectively). Although renowned inhibitors, such as acetylsalicylic acid and quercetin showed higher activity, this study verifies P. lanceolata and P. major as considerable anti-inflammatory agents. https://greenmedinfo.com/article/vitro-test-cyclooxygenase-1-cox-1-and-12-lipoxygenase-12-lox-inhibition-potenc#comments Inflammation Plantago lanceolata Plantago major 12-lipoxygenase (12-LOX) Anti-Inflammatory Agents Cyclooxygenase 1 Inhibitor Cyclooxygenase Inhibitors Plant Extracts In Vitro Study Sat, 30 Jun 2012 21:42:00 +0000 greenmedinfo 77884 at https://greenmedinfo.com Stinging nettle is an interesting candidate for co-treatment of various inflammatory diseases. https://greenmedinfo.com/article/stinging-nettle-interesting-candidate-co-treatment-various-inflammatory-diseas PMID:  Phytother Res. 2017 May 24. Epub 2017 May 24. PMID: 28544187 Abstract Title:  Chemical Composition and Immuno-Modulatory Effects of Urtica dioica L. (Stinging Nettle) Extracts. Abstract:  The purpose of this work was to determine the chemical profile of stinging nettle and to provide an insight into the mechanisms by which it ameliorates the immune response. Qualitative and quantitative liquid chromatography tandem mass spectrometry analyses indicated that phenolic acids (5-O-caffeoylquinic acid as dominant) and flavonol glycosides (rutin, isoquercitrin, and kaempferol 3-O-glucoside) are present in the aerial parts, while lignans (secoisolariciresinol, 9,9&#039;-bisacetyl-neo-olivil and their glucosides) were detected in the root. Herb and root extracts expressed selective inhibition toward cyclooxygenase and lipoxygenase branches in human platelets: root extracts were better at inhibiting thromboxane production, while herb extracts were more specific toward inhibition of 12-lipoxygenase pathway. Stinging nettle extracts mildly increased monocyte chemoattractant protein-1 and growth-related oncogene release from nonstimulated intestinal epithelial cells, stimulating MyD88/NF-κB/p38 signaling, hence preserving the epithelial integrity and enhancing intestinal steady-state defense. Additionally, root extract reduced lipopolysaccharide-induced monocyte chemoattractant protein-1/growth-related oncogene secretion and cyclooxygenase-2 expression in intestinal epithelial cells, thus showing the potential protective effect against tissue damage caused by inflammation processes. These observations suggest that stinging nettle is an interesting candidate for the development of phytopharmaceuticals or dietary supplements for cotreatment of various inflammatory diseases, particularly inflammatory bowel diseases. Copyright © 2017 John Wiley&amp;Sons, Ltd. <p><a href="https://greenmedinfo.com/article/stinging-nettle-interesting-candidate-co-treatment-various-inflammatory-diseas" target="_blank">read more</a></p> https://greenmedinfo.com/article/stinging-nettle-interesting-candidate-co-treatment-various-inflammatory-diseas#comments Inflammation Inflammatory Bowel Diseases Nettle 12-lipoxygenase (12-LOX) Anti-Inflammatory Agents Cyclooxygenase 2 Inhibitors In Vitro Study Wed, 31 May 2017 21:52:05 +0000 greenmedinfo 148489 at https://greenmedinfo.com