Gene Exprsession Regulation https://greenmedinfo.com/category/keywords/Gene%20Exprsession%20Regulation en Active ingredients and mechanisms of Phellinus linteus for alleviation of Type 2 diabetes mellitus. https://greenmedinfo.com/article/active-ingredients-and-mechanisms-phellinus-linteus-alleviation-type-2-diabete PMID:  Gene. 2021 Feb 5 ;768:145320. Epub 2020 Nov 26. PMID: 33248199 Abstract Title:  Active ingredients and mechanisms of Phellinus linteus (grown on Rosa multiflora) for alleviation of Type 2 diabetes mellitus through network pharmacology. Abstract:  Phellinus linteus (mushroom) grown on Rosa multiflora (PL@RM), exposed beneficial effect and safety on Type 2 diabetes mellitus (T2DM) from Korean folk remedies. However, its active chemical constituents and mechanism(s) against T2DM have not been confirmed. Hence, we deciphered the active compounds and mechanism(s) of PL@RM against T2DM through network pharmacology. GC-MS of PL@RM manifested 54 compounds and drug-likeness properties of these compounds were confirmed by Lipinski&#039;s rule. The compound (40) related genes were composed of Similarity Ensemble Approach (SEA) and SwissTargetPrediction (STP). The overlapping genes (61) between the two databases were identified. Besides, the T2DM related genes (4,736) were extracted from DisGeNet and OMIM database. In parallel, a Venn diagram was constructed between the overlapping genes (61) and T2DM related genes (4,736), and finally, 48 genes were picked. The interactive networks between compounds and overlapping genes were plotted and visualized by RStudio. In addition, KEGG Pathway enrichment analysis was evaluated by String. String analysis showed that the mechanisms of PL@RM against T2DM were related to 16 pathways, where inhibition of gluconeogenesis by inactivating metabolic pathways was noted as the hub pathway of PL@RM against T2DM. Besides, bubble chart indicated that activation of the AMPK signaling pathway might enhance the insulin receptor (IR) phosphorylation, which is regarded the key signaling pathway of PL@RM against T2DM. Furthermore, the autodock vina revealed the promising binding affinity energy of the epicholesterol (the most drug-likeness compound) on HMGCR (hub gene). Overall, this work hints at the therapeutic evidence of PL@RM on T2DM, and this data expound the main chemical compounds and mechanisms of PL@RM against T2DM. <p><a href="https://greenmedinfo.com/article/active-ingredients-and-mechanisms-phellinus-linteus-alleviation-type-2-diabete" target="_blank">read more</a></p> https://greenmedinfo.com/article/active-ingredients-and-mechanisms-phellinus-linteus-alleviation-type-2-diabete#comments Diabetes Mellitus: Type 2 Phellinus Linteus Gene Exprsession Regulation In Vitro Study Tue, 06 Jul 2021 19:51:04 +0000 greenmedinfo 242266 at https://greenmedinfo.com Fasting selectively blocks development of acute lymphoblastic leukemia. https://greenmedinfo.com/article/fasting-selectively-blocks-development-acute-lymphoblastic-leukemia n/a PMID:  Nat Med. 2017 Jan ;23(1):79-90. Epub 2016 Dec 12. PMID: 27941793 Abstract Title:  Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation. Abstract:  New therapeutic approaches are needed to treat leukemia effectively. Dietary restriction regimens, including fasting, have been considered for the prevention and treatment of certain solid tumor types. However, whether and how dietary restriction affects hematopoietic malignancies is unknown. Here we report that fasting alone robustly inhibits the initiation and reverses the leukemic progression of both B cell and T cell acute lymphoblastic leukemia (B-ALL and T-ALL, respectively), but not acute myeloid leukemia (AML), in mouse models of these tumors. Mechanistically, we found that attenuated leptin-receptor (LEPR) expression is essential for the development and maintenance of ALL, and that fasting inhibits ALL development by upregulation of LEPR and its downstream signaling through the protein PR/SET domain 1 (PRDM1). The expression of LEPR signaling-related genes correlated with the prognosis of pediatric patients with pre-B-ALL, and fasting effectively inhibited B-ALL growth in a human xenograft model. Our results indicate that the effects of fasting on tumor growth are cancer-type dependent, and they suggest new avenues for the development of treatment strategies for leukemia. https://greenmedinfo.com/article/fasting-selectively-blocks-development-acute-lymphoblastic-leukemia#comments Acute lymphoblastic leukemia (ALL) Acute T cell Leukemias Antiproliferative Chemopreventive Fasting/Caloric Restriction Acute lymphoblastic leukemia (ALL) Acute T cell Leukemias Antiproliferative Chemopreventive Fasting/Caloric Restriction Gene Exprsession Regulation Selective Antiproliferation Animal Study Tue, 17 Jan 2017 03:57:26 +0000 greenmedinfo 142072 at https://greenmedinfo.com The results of the current study provide a theoretical basis for hyperoside treatment of cervical cancer. https://greenmedinfo.com/article/results-current-study-provide-theoretical-basis-hyperoside-treatment-cervical- PMID:  Cancer Cell Int. 2019 ;19:235. Epub 2019 Sep 9. PMID: 31516392 Abstract Title:  Effect of hyperoside on cervical cancer cells and transcriptome analysis of differentially expressed genes. Abstract:  Background: Hyperoside (Hy) is a plant-derived quercetin 3-d-galactoside that exhibits inhibitory activities on various tumor types. The objective of the current study was to explore Hy effects on cervical cancer cell proliferation, and to perform a transcriptome analysis of differentially expressed genes.Methods: Cervical cancer HeLa and C-33A cells were cultured and the effect of Hy treatment was determined using the Cell Counting Kit-8 (CCK-8) assay. After calculating the IC50 of Hy in HeLa and C-33A cells, the more sensitive to Hy treatment cell type was selected for RNA-Seq. Differentially expressed genes (DEGs) were identified by comparing gene expression between the Hy and control groups. Candidate genes were determined through DEG analysis, protein interaction network (PPI) construction, PPI module analysis, transcription factor (TF) prediction, TF-target network construction, and survival analysis. Finally, the key candidate genes were verified by RT-qPCR and western blot.Results: Hy inhibited HeLa and C33A cell proliferation in a dose- and time-dependent manner, as determined by the CCK-8 assay. Treatment of C-33A cells with 2 mM Hy was selected for the subsequent experiments. Compared with the control group, 754 upregulated and 509 downregulated genes were identified after RNA-Seq. After functional enrichment, 74 gene ontology biological processes and 43 Kyoto Encyclopedia of Genes and Genomes pathways were obtained. According to the protein interaction network (PPI), PPI module analysis, TF-target network construction, and survival analysis, the key genes,,,,,,,,,,,, andwere detected. RT-qPCR was performed on the key genes, and Western blot was used to verify-and.-andexpressions were lower and higher than the corresponding values in the control group, respectively, in accordance with the results from the RNA-Seq analysis.Conclusion: Hy inhibited HeLa and C-33A cell proliferation through-gene expression reduction in C-33A cells andregulation. The results of the current study provide a theoretical basis for Hy treatment of cervical cancer. <p><a href="https://greenmedinfo.com/article/results-current-study-provide-theoretical-basis-hyperoside-treatment-cervical-" target="_blank">read more</a></p> https://greenmedinfo.com/article/results-current-study-provide-theoretical-basis-hyperoside-treatment-cervical-#comments Cervical Cancer Flavonoids Antiproliferative Gene Exprsession Regulation In Vitro Study Thu, 22 Jul 2021 17:31:14 +0000 greenmedinfo 243067 at https://greenmedinfo.com