Gastroprotective https://greenmedinfo.com/category/keywords/Gastroprotective en Gastroprotective activities of adlay on the growth of the stomach cancer AGS cell line and indomethacin-induced gastric ulcers. https://greenmedinfo.com/article/gastroprotective-activities-adlay-growth-stomach-cancer-ags-cell-line-and-indo n/a PMID:  J Agric Food Chem. 2011 Jun 8 ;59(11):6025-33. Epub 2011 May 5. PMID: 21517098 Abstract Title:  Gastroprotective activities of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) on the growth of the stomach cancer AGS cell line and indomethacin-induced gastric ulcers. Abstract:  Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seeds have long been used to treat warts, chapped skin, rheumatism, and neuralgia in traditional Chinese medicine (TCM). Recently, studies demonstrated its anti-inflammatory, antiproliferative, antitumor, and antiallergic activities. In the present study, we first report the gastroprotective effects of dehulled adlay (DA) seeds, which consist of bran (AB) and endosperm (AE). The DA ethanolic extract (DAE) was prepared, along with the AB and AE ethanolic extracts (ABE and AEE), and the inhibitory effects of these extracts were tested on the AGS gastric cancer cell line. Results indicated that the ABE showed better antiproliferative activity, and 19 compounds were purified from AB in a further phenolic-compound-guided separation. Among the isolated compounds, caffeic and chlorogenic acids significantly suppressed the growth of AGS cells. In addition, the antiulcer activity of DA was examined in an indomethacin-induced gastric lesion model. The ulcer index (UI) and oxidative biomarkers in animals decreased, while the non-protein sulfhydryl (NPSH) groups were elevated when given DA. This is the first investigation of antiulcer activity of adlay, and we demonstrated that the antioxidative-active phenolic acids in DA contribute to some portion of the gastroprotective effects. https://greenmedinfo.com/article/gastroprotective-activities-adlay-growth-stomach-cancer-ags-cell-line-and-indo#comments Adlay Gastric Cancer Gastric Ulcer Antioxidants Antiproliferative Gastroprotective Adlay Antioxidants Antiproliferative gastric cancer Gastric Ulcer Gastroprotective Plant Extracts Animal Study In Vitro Study Sat, 21 Apr 2018 00:33:07 +0000 greenmedinfo 163111 at https://greenmedinfo.com Lactobacillus reuteri strains protect epithelial barrier integrity from the detrimental effect of enterotoxigenic Escherichia coli. https://greenmedinfo.com/article/lactobacillus-reuteri-strains-protect-epithelial-barrier-integrity-detrimental n/a PMID:  Physiol Rep. 2018 Jan ;6(2). PMID: 29368445 Abstract Title:  Lactobacillus reuteri strains protect epithelial barrier integrity of IPEC-J2 monolayers from the detrimental effect of enterotoxigenic Escherichia coli. Abstract:  Lactobacillus reuteri is an inhabitant of the gastrointestinal (GI) tract of mammals and birds and several strains of this species are known to be effective probiotics. The mechanisms by which L. reuteri confers its health-promoting effects are far from being fully understood, but protection of the mucosal barrier is thought to be important. Leaky gut is a state of abnormal intestinal permeability with implications for the pathophysiology of various gastrointestinal disorders. Enterotoxigenic Escherichia coli (ETEC) can invade the intestinal mucosa and induce changes in barrier function by producing enterotoxin or by direct invasion of the intestinal epithelium. Our hypothesis was that L. reuteri can protect the mucosal barrier, and the goal of the study was to challenge this hypothesis by monitoring the protective effect of L. reuteri strains on epithelial dysfunction caused by ETEC. Using an infection model based on the porcine intestinal cell line IPEC-J2, it was demonstrated that pretreatment of the cells with human-derived L. reuteri strains (ATCC PTA 6475, DSM 17938 and 1563F) and a rat strain (R2LC) reduced the detrimental effect of ETEC in a dose-dependent manner, as monitored by permeability of FITC-dextran and transepithelial electrical resistance (TEER). Moreover, the results revealed that ETEC upregulated proinflammatory cytokines IL-6 and TNFα and decreased expression of the shorter isoform of ZO-1 (187 kDa) and E-cadherin. In contrast, pretreatment with L. reuteri DSM 17938 and 1563F downregulated expression of IL-6 and TNFα, and led to an increase in production of the longer isoform of ZO-1 (195 kDa) and maintained E-cadherin expression. Interestingly, expression of ZO-1 (187 kDa) was preserved only when the infected cells were pretreated with strain 1563F. These findings demonstrate that L. reuteri strains exert a protective effect against ETEC-induced mucosal integrity disruption. https://greenmedinfo.com/article/lactobacillus-reuteri-strains-protect-epithelial-barrier-integrity-detrimental#comments Escherichia coli Infections Lactobacillus reuteri Anti-Inflammatory Agents Gastroprotective Interleukin-6 Downregulation Tumor Necrosis Factor (TNF) Alpha Inhibitor Anti-Inflammatory Agents Escherichia coli Infections Gastroprotective Lactobacillus reuteri Animal Study Sat, 27 Jan 2018 03:49:40 +0000 greenmedinfo 158905 at https://greenmedinfo.com Nigella sativa oil may help prevent the accompanying gastrointestinal dysfunction in cisplatin chemotherapy. https://greenmedinfo.com/article/nigella-sativa-oil-may-help-prevent-accompanying-gastrointestinal-dysfunction- n/a PMID:  Exp Toxicol Pathol. 2017 Feb 15. Epub 2017 Feb 15. PMID: 28215571 Abstract Title:  Oral administration of Nigella sativa oil ameliorates the effect of cisplatin on brush border membrane enzymes, carbohydrate metabolism and antioxidant system in rat intestine. Abstract:  Cisplatin (CP) is an effective chemotherapeutic agent that induces gastrointestinal toxicity. Nigella sativa oil (NSO) has been shown to be beneficial in a wide range of gastrointestinal disorders. The present study investigates the possible protective effect of NSO on CP-induced gastrointestinal toxicity. NSO administration (2ml/kg bwt, orally), prior to and following, a single dose CP treatment (6mg/kg bwt. ip), significantly attenuated the CP-induced decrease in brush border membrane (BBM) enzyme activities in intestinal homogenates and BBM vesicles (BBMV). NSO administration also mitigated CP induced alterations in the activities of carbohydrate metabolism enzymes and in the enzymatic and non-enzymatic antioxidant parameters in the intestine. The results suggest that NSO by empowering the endogenous antioxidant system improves intestinal redox and metabolic status and restores BBM integrity in CP treated rats. Histopathological studies supported the biochemical findings. Thus, NSO may help prevent the accompanying gastrointestinal dysfunction in CP chemotherapy. https://greenmedinfo.com/article/nigella-sativa-oil-may-help-prevent-accompanying-gastrointestinal-dysfunction-#comments Chemotherapy-Induced Toxicity: Cisplatin Nigella sativa (aka Black Seed) Chemoprotective Agents Gastroprotective Chemoprotective Agents Chemotherapy-Induced Toxicity: Cisplatin Gastroprotective Nigella sativa (aka Black Seed) Animal Study Wed, 15 Mar 2017 20:44:42 +0000 greenmedinfo 144868 at https://greenmedinfo.com