Radioprotective https://greenmedinfo.com/category/keywords/Radioprotective en Curcumin protects against gamma ray induced conformational change of human serum albumin. https://greenmedinfo.com/article/curcumin-protects-against-gamma-ray-induced-conformational-change-human-serum- n/a PMID:  Int J Biol Macromol. 2017 Jun ;99:600-607. Epub 2017 Mar 6. PMID: 28274864 Abstract Title:  Protective effects of curcumin against gamma ray induced conformational change of human serum albumin. Abstract:  This study explores the possibility for protection by curcumin during the molecular and structural changes of human serum albumin (HSA) exposed to gamma irradiation. We used a combination of spectroscopic methods to probe the conformational ensemble of the irradiated HSA and finally evaluated the extent of restoration by curcumin. SDS-PAGE study unfolded the formation of cross linked aggregates as a consequence of increasing exposure of gamma radiation. CD and FTIR spectroscopy indicated significant decrease in the alpha helix content of HSA from 57% to 15% with increasing radiation doses. Steady state and time resolved fluorescence studies complemented the spectroscopic measurements, when lifetime decay was significantly reduced from 6.35ns to 0.37ns. Hydrophobic study showed the effectiveness of curcumin for protection at low dose of gamma irradiated HSA samples. We integrated these observations to investigate protein aggregation under increasing gamma radiation and estimated the same in presence of curcumin. It was elucidated, that when HSA is irradiated at low dose of gamma radiation in presence of curcumin, it is capable of retaining the characteristic properties to a higher extent indicating stabilization of molecular structure of HSA by curcumin. A model for curcumin based protection has been proposed utilizing ThT assay. https://greenmedinfo.com/article/curcumin-protects-against-gamma-ray-induced-conformational-change-human-serum-#comments Curcumin Radiation Induced Illness Antioxidants Radioprotective Antioxidants CURCUMIN Radiation Induced Illness Radioprotective In Vitro Study Tue, 16 May 2017 22:25:09 +0000 greenmedinfo 147822 at https://greenmedinfo.com Hippophae extract counters neuronal tissue injuries and changes in neurotransmitters: implications in radiation protection. https://greenmedinfo.com/article/hippophae-extract-counters-neuronal-tissue-injuries-and-changes-neurotransmitt n/a PMID:  Pharm Biol. 2017 Dec ;55(1):1833-1842. PMID: 28552029 Abstract Title:  A standardized Hippophae extract (SBL-1) counters neuronal tissue injuries and changes in neurotransmitters: implications in radiation protection. Abstract:  CONTEXT: Effects of a radioprotective, standardized leaf extract (code SBL-1) from traditional medicinal plant, sea buckthorn [Hippophae rhamnoides L. (Elaeagnaceae)], on neurotransmitters and brain injuries in rats showing radiation-induced conditioned taste aversion (CTA), are not known. Understanding CTA in rats is important because its process is considered parallel to nausea and vomiting in humans. OBJECTIVE: This study investigated the levels of neurotransmitters, antioxidant defences and histological changes in rats showing radiation CTA, and their modification by SBL-1. MATERIALS AND METHODS: The inbred male Sprague-Dawley rats (age 65 days, weighing 190 ± 10 g) were used. Saccharin-preferring rats were selected using standard procedure and divided into groups. Group I (untreated control) was administered sterile water, group II wasCo-γ-irradiated (2 Gy), and group III was administered SBL-1 before irradiation. Observations were recorded up to day 5. RESULTS: Irradiation (2 Gy) caused (i) non-recoverable CTA (≥ 64.7 ± 5.0%); (ii) degenerative changes in cerebral cortex, amygdala and hippocampus; (iii) increases in brain dopamine (DA, 63.4%), norepinephrine (NE, 157%), epinephrine (E, 233%), plasma NE (103%) and E (160%); and (iv) decreases in brain superoxide dismutase (67%), catalase (60%) and glutathione (51%). SBL-1 treatment (12 mg/kg body weight) 30 min before irradiation (i) countered brain injuries, (ii) reduced CTA (38.7 ± 3.0%, day 1) and (iii) normalized brain DA, NE, E, superoxide dismutase, catalase and CTA from day 3 onwards. DISCUSSION AND CONCLUSION: Radiation CTA was coupled with brain injuries, disturbances in neurotransmitters and antioxidant defences. SBL-1 pretreatment countered these disturbances, indicating neuroprotective action. https://greenmedinfo.com/article/hippophae-extract-counters-neuronal-tissue-injuries-and-changes-neurotransmitt#comments Chemotherapy and Radiation Toxicity Oxidative Stress Sea buckthorn Antioxidants Neuroprotective Agents Radioprotective Antioxidants Chemotherapy and Radiation Toxicity Neuroprotective Agents oxidative stress Plant Extracts Radioprotective Sea buckthorn Animal Study Sat, 07 Apr 2018 16:03:58 +0000 greenmedinfo 162347 at https://greenmedinfo.com These results demonstrat the radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability in vitro. https://greenmedinfo.com/article/these-results-demonstrat-radioprotective-effect-folic-acid-supplementation-low n/a PMID:  Indian J Exp Biol. 2016 08 ;54(8):537-43. PMID: 28577512 Abstract Title:  Possible radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability in vitro. Abstract:  Ionizing radiation (IR) induces DNA damage through production of single and double-strand breaks and reactive oxygen species (ROS). Folic acid (FA) prevents radiation-induced DNA damage by modification of DNA synthesis and/or repair and as a radical scavenger. We hypothesized that in vitro supplementation with FA will decrease the sensitivity of cells to genetic damage induced by low dose of ionizing radiation. Annexin V, comet and micronucleus assays were performed in cultured CHO cells. After 7 days of pre-treatment with 0, 100, 200 or 300 nM FA, cultures were exposed to radiation (100 mSv). Two un-irradiated controls were executed (0 and 100 nM FA). Data were statistically analyzed with X2-test and linear regression analysis (P0.05). We observed a significantly decreased frequency of apoptotic cells with the increasing FA concentration (P<0.05). The same trend was observed when analyzing DNA damage and chromosomal instability (P<0.05 for 300 nM). Only micronuclei frequencies showed significant differences for linear regression analysis (R2=94.04; P<0.01). Our results have demonstrated the radioprotective effect of folic acid supplementation on low dose ionizing radiation-induced genomic instability in vitro; folate status should be taken into account when studying the effect of low dose radiation in environmental or occupational exposure. https://greenmedinfo.com/article/these-results-demonstrat-radioprotective-effect-folic-acid-supplementation-low#comments DNA damage Folic Acid Anti-Apoptotic Low Dose Ionizing Radiation Radioprotective Anti-Apoptotic DNA damage folic acid Radioprotective In Vitro Study Wed, 05 Jul 2017 23:23:44 +0000 greenmedinfo 150102 at https://greenmedinfo.com