Bisphenol F https://greenmedinfo.com/category/keywords/Bisphenol%20F en BPA alternatives are not necessarily less estrogenic than BPA in human breast cancer cells. https://greenmedinfo.com/article/bpa-alternatives-are-not-necessarily-less-estrogenic-bpa-human-breast-cancer-c n/a PMID:  Toxicol Sci. 2017 Jun 7. Epub 2017 Jun 7. PMID: 28591870 Abstract Title:  Transcriptome profiling reveals bisphenol A alternatives activate estrogen receptor alpha in human breast cancer cells. Abstract:  Plasticizers with estrogenic activity, such as bisphenol A (BPA), have potential adverse health effects in humans. Due to mounting evidence of these health effects, BPA is being phased out and replaced by other bisphenol variants in"BPA-free"products. We have compared estrogenic activity of BPA to 6 bisphenol analogues (bisphenol S, BPS; bisphenol F, BPF; bisphenol AP, BPAP; bisphenol AF, BPAF; bisphenol Z, BPZ; bisphenol B, BPB) in three human breast cancer cell lines. Estrogenicity was assessed (10-11M to 10-4M) by cell growth in an estrogen receptor (ER)-mediated cell proliferation assay, and by the induction of estrogen response element-mediated transcription in a luciferase assay. BPAF was the most potent bisphenol, followed by BPB>BPZ∼ BPA>BPF∼ BPAP>BPS. The addition of ICI 182,780 antagonized the activation of ERs. Data mining of ToxCast high-throughput screening assays confirms our results but also shows divergence in the sensitivities of the assays. Gene expression profiles were determined in MCF-7 cells by microarray analysis. The comparison of transcriptome profile alterations resulting from BPA alternatives with an ERα gene expression biomarker further indicates that all BPA alternatives act as ERα agonists in MCF-7 cells. These results were confirmed by Illumina-based RNA sequencing. In conclusion, BPA alternatives are not necessarily less estrogenic than BPA in human breast cancer cells. BPAF, BPB, and BPZ were more estrogenic than BPA. These findings point to the importance of better understanding the risk of adverse effects from exposure to BPA alternatives, including hormone-dependent breast cancer. https://greenmedinfo.com/article/bpa-alternatives-are-not-necessarily-less-estrogenic-bpa-human-breast-cancer-c#comments Bisphenol Toxicity Breast Cancer Bisphenol A Bisphenol AP Bisphenol F Bisphenol S Bisphenol A Bisphenol AP Bisphenol F Bisphenol S Bisphenol Toxicity Breast Cancer In Vitro Study Fri, 18 Aug 2017 00:57:31 +0000 greenmedinfo 151849 at https://greenmedinfo.com Low-dose exposure to bisphenols A, F and S of human primary adipocyte impacts coding and non-coding RNA profiles. https://greenmedinfo.com/article/low-dose-exposure-bisphenols-f-and-s-human-primary-adipocyte-impacts-coding-an n/a PMID:  PLoS One. 2017 ;12(6):e0179583. Epub 2017 Jun 19. PMID: 28628672 Abstract Title:  Low-dose exposure to bisphenols A, F and S of human primary adipocyte impacts coding and non-coding RNA profiles. Abstract:  Bisphenol A (BPA) exposure has been suspected to be associated with deleterious effects on health including obesity and metabolically-linked diseases. Although bisphenols F (BPF) and S (BPS) are BPA structural analogs commonly used in many marketed products as a replacement for BPA, only sparse toxicological data are available yet. Our objective was to comprehensively characterize bisphenols gene targets in a human primary adipocyte model, in order to determine whether they may induce cellular dysfunction, using chronic exposure at two concentrations: a"low-dose"similar to the dose usually encountered in human biological fluids and a higher dose. Therefore, BPA, BPF and BPS have been added at 10 nM or 10μM during the differentiation of human primary adipocytes from subcutaneous fat of three non-diabetic Caucasian female patients. Gene expression (mRNA/lncRNA) arrays and microRNA arrays, have been used to assess coding and non-coding RNA changes. We detected significantly deregulated mRNA/lncRNA and miRNA at low and high doses. Enrichment in"cancer"and"organismal injury and abnormalities"related pathways was found in response to the three products. Some long intergenic non-coding RNAs and small nucleolar RNAs were differentially expressed suggesting that bisphenols may also activate multiple cellular processes and epigenetic modifications. The analysis of upstream regulators of deregulated genes highlighted hormones or hormone-like chemicals suggesting that BPS and BPF can be suspected to interfere, just like BPA, with hormonal regulation and have to be considered as endocrine disruptors. All these results suggest that as BPA, its substitutes BPS and BPF should be used with the same restrictions. https://greenmedinfo.com/article/low-dose-exposure-bisphenols-f-and-s-human-primary-adipocyte-impacts-coding-an#comments Bisphenol Toxicity Bisphenol A Bisphenol F Bisphenol S Bisphenol A Bisphenol F Bisphenol S Bisphenol Toxicity Human In Vitro Fri, 18 Aug 2017 00:44:56 +0000 greenmedinfo 151846 at https://greenmedinfo.com