Sembung Leaf https://greenmedinfo.com/category/keywords/Sembung%20Leaf en Effects and mechanisms of total flavonoids from Blumea balsamifera on skin wound in rats. https://greenmedinfo.com/article/effects-and-mechanisms-total-flavonoids-blumea-balsamifera-skin-wound-rats n/a PMID:  Int J Mol Sci. 2017 Dec 19 ;18(12). Epub 2017 Dec 19. PMID: 29257119 Abstract Title:  Effects and Mechanisms of Total Flavonoids from Blumea balsamifera (L.) DC. on Skin Wound in Rats. Abstract:  Chinese herbal medicine (CHM) evolved through thousands of years of practice and was popular not only among the Chinese population, but also most countries in the world.(L.) DC. as a traditional treatment for wound healing in Li Nationality Medicine has a long history of nearly 2000 years. This study was to evaluate the effects of total flavonoids from(L.) DC. on skin excisional wound on the back of Sprague-Dawley rats, reveal its chemical constitution, and postulate its action mechanism. The rats were divided into five groups and the model groups were treated with 30% glycerol, the positive control groups with Jing Wan Hong (JWH) ointment, and three treatment groups with high dose (2.52 g·kg), medium dose (1.26 g·kg), and low dose (0.63 g·kg) of total flavonoids from. During 10 consecutive days of treatment, the therapeutic effects of rates were evaluated. On day 1, day 3, day 5, day 7, and day 10 after treatment, skin samples were taken from all the rats for further study. Significant increases of granulation tissue, fibroblast, and capillary vessel proliferation were observed at day 7 in the high dose and positive control groups, compared with the model group, with the method of 4% paraformaldehyde for histopathological examination and immunofluorescence staining. To reveal the action mechanisms of total flavonoids on wound healing, the levels of CD68, vascular endothelial growth factor (VEGF), transforming growth factor-₁ (TGF-₁), and hydroxyproline were measured at different days. Results showed that total flavonoids had significant effects on rat skin excisional wound healing compared with controls, especially high dose ones (<0.05). Furthermore, the total flavonoid extract was investigated phytochemically, and twenty-seven compounds were identified from the total flavonoid sample by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry/diode array detector (UPLC-Q-TOF-MS/DAD), including 16 flavonoid aglucons, five flavonoid glycosides (main peaks in chromatogram), five chlorogenic acid analogs, and 1 coumarin. Reports show that flavonoid glycoside possesses therapeutic effects of curing wounds by inducing neovascularization, and chlorogenic acid also has anti-inflammatory and wound healing activities; we postulated that all the ingredients in total flavonoids sample maybe exert a synergetic effect on wound curing. Accompanied with detection of four growth factors, the upregulation of these key growth factors may be the mechanism of therapeutic activities of total flavonoids. The present study confirmed undoubtedly that flavonoids were the main active constituents that contribute to excisional wound healing, and suggested its action mechanism of improving expression levels of growth factors at different healing phases. https://greenmedinfo.com/article/effects-and-mechanisms-total-flavonoids-blumea-balsamifera-skin-wound-rats#comments Sembung Leaf Wound Healing Anti-Inflammatory Agents Anti-Inflammatory Agents Sembung Leaf wound healing Animal Study Sat, 07 Apr 2018 12:48:34 +0000 greenmedinfo 162325 at https://greenmedinfo.com Nitric oxide inhibitory constituents as potential anti-neuroinflammatory agents for Alzheimer's disease from Blumea balsamifera. https://greenmedinfo.com/article/nitric-oxide-inhibitory-constituents-potential-anti-neuroinflammatory-agents-a n/a PMID:  Bioorg Chem. 2018 Feb ;76:449-457. Epub 2017 Dec 5. PMID: 29275263 Abstract Title:  NO inhibitory constituents as potential anti-neuroinflammatory agents for AD from Blumea balsamifera. Abstract:  Our continuous search for new nitric oxide (NO) inhibitory substances as anti-neuroinflammatory agents for AD resulted in the isolation of one new labdane diterpenoid and three new guaiane sesquiterpenoids, as well as ten known compounds from Blumea balsamifera. Their structures were elucidated by NMR spectroscopic data analysis and the time-dependent density functional theory (TDDFT) electronic circular dichroism (ECD) calculations. The anti-neuroinflammatory effects were examined by inhibiting NO release in LPS-induced murine microglial BV-2 cells. The possible mechanism of NO inhibition of some bioactive compounds was also investigated using molecular docking, which revealed the interactions of bioactive compounds with the iNOS protein. https://greenmedinfo.com/article/nitric-oxide-inhibitory-constituents-potential-anti-neuroinflammatory-agents-a#comments Alzheimer's Disease Brain Inflammation Sembung Leaf Anti-Inflammatory Agents Nitric Oxide Inhibitor Alzheimer's disease Anti-Inflammatory Agents Brain Inflammation Nitric Oxide Inhibitor Sembung Leaf In Vitro Study Sat, 07 Apr 2018 12:29:47 +0000 greenmedinfo 162323 at https://greenmedinfo.com