Bodily Tissue Regeneration https://greenmedinfo.com/category/keywords/Bodily%20Tissue%20Regeneration en 6 Bodily Tissues That Can Be Regenerated Through Nutrition https://greenmedinfo.com/blog/6-bodily-tissues-can-be-regenerated-through-nutrition2 <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2023<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="6 Bodily Tissues That Can Be Regenerated Through Nutrition" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/greenmedinfo/images/tissue_regeneration(1).jpg" style="width: 300px; height: 400px;" title="6 Bodily Tissues That Can Be Regenerated Through Nutrition" /></p> <p><span style="font-size:20px;"><em><strong>It may come as a surprise to some, especially those with conventional medical training, but the default state of the body is one of ceaseless <a href="/blog/flame-cell-life-death-miracle-regeneration" rel="dofollow" target="_blank">regeneration</a>. Without the flame-like process of continual cell turnover within the body - life and death ceaselessly intertwined - the miracle of the human body would not exist</strong></em></span></p><p><a href="https://greenmedinfo.com/blog/6-bodily-tissues-can-be-regenerated-through-nutrition2" target="_blank">read more</a></p> https://greenmedinfo.com/blog/6-bodily-tissues-can-be-regenerated-through-nutrition2#comments Brain Damage Curcumin Demyelinating Diseases Glycyrrhizin Neurodegenerative Diseases Osteoarthritis Resveratrol Spinal Cord Injuries Stevia Trigonelline Vitamin C Blood Sugar Problems Health Guide: Aging Heart Health Neuritogenic Secretagogue Beta Cell Regeneration Bodily Tissue Regeneration Bone Regeneration Hormone Regeneration Liver Regeneration Myocardial Regeneration natural Bodily Tissue Regeneration Regenerative Actions Regenerative Substances Remyelination Sat, 29 Oct 2022 00:03:34 +0000 Sayer Ji 75902 at https://greenmedinfo.com Berberine promotes osteogenic differentiation of mesenchymal stem cells with therapeutic potential in periodontal regeneration. https://greenmedinfo.com/article/berberine-promotes-osteogenic-differentiation-mesenchymal-stem-cells-therapeut PMID:  Eur J Pharmacol. 2019 Feb 15. Epub 2019 Feb 15. PMID: 30776366 Abstract Title:  Berberine promotes osteogenic differentiation of mesenchymal stem cells with therapeutic potential in periodontal regeneration. Abstract:  Periodontal disease is a bacterial infection-associated disease of the periodontal tissues characterized by the destruction of tooth-supporting structures, including alveolar bone. The ideal goal of periodontal therapy is the complete regeneration of alveolar bone in a healthy microenvironment free of infection. In this study, we found that berberine, a benzylisoquinoline plant alkaloid from Coptidis Rhizoma, strongly inhibited the growth of Porphyromonas gingivalis. Gingipain is the most important virulence factor of Porphyromonas gingivalis in the process of periodontal tissue destruction. Berberine also had an inhibitory effect on gingipain activity in a concentration dependent manner. Remarkably, berberine restored the downregulation of osteogenesis-related genes expression in bone mesenchymal stem cells (BMSCs) induced by Porphyromonas gingivalis infection,and significantly increased the expression of osteogenesis-related genes such as OSX, COLI, ALP, OCN and OPN compared to the control group. This results suggested that berberine may directly promote osteogenesis. Further in-vitro studies demonstrated that berberine statistically significantly promoted the osteogenic differentiation of BMSCs at concentrations of 1 to 10 μM. In the research on the mechanisms, we found that both total β-catenin and nuclear β-catenin accumulation were statistically significantly increased by berberine. And the transcriptional activity of β-catenin/TCF was about 2 folds higher than the control group. Furthermore, Wnt signaling specific inhibitor DKK-1 blocked the above effects of berberine. These demonstrated that Wnt/β-catenin signalling pathway was involved in the osteogenic differentiation induced by berberine. The antibacterial actions in combinationwith the promotion role in osteogenic differentiation position berberine as a prospective drug for periodontal tissue regeneration. <p><a href="https://greenmedinfo.com/article/berberine-promotes-osteogenic-differentiation-mesenchymal-stem-cells-therapeut" target="_blank">read more</a></p> https://greenmedinfo.com/article/berberine-promotes-osteogenic-differentiation-mesenchymal-stem-cells-therapeut#comments Berberine Periodontal Diseases Regenerative Wnt/β-catenin signaling pathway modulation Bodily Tissue Regeneration Mesenchymal Stem Cells In Vitro Study Fri, 01 Mar 2019 00:41:01 +0000 greenmedinfo 180482 at https://greenmedinfo.com Electroacupuncture of Weizhong (BL 40) acupoints can restore aged back muscular satellite cells and their regeneration capacity. https://greenmedinfo.com/article/electroacupuncture-weizhong-bl-40-acupoints-can-restore-aged-back-muscular-sat PMID:  Evid Based Complement Alternat Med. 2022 ;2022:2695679. Epub 2022 Aug 4. PMID: 35966754 Abstract Title:  Electroacupuncture of Weizhong (BL-40) Acupoint Inspires Muscular Satellite Cell Regeneration and Promotes Muscle Repair Capacity after Back Muscle Injury in Sprague-Dawley Rat Model. Abstract:  Background: Back muscle injury is the most common illness involved in aged people. Muscular satellite cells, playing a key role in the muscle repairing process, are gradually losing their regenerative ability with aging, which attenuates the injured muscle repairing process. Electroacupuncture at Weizhong acupoint has been widely used in the treatment of young and aged patients with back muscle damage. Its efficacy has been proven by a randomized double-blind placebo clinical trial. However, the rehabilitation mechanisms are largely unknown. This study will explore the possible mechanisms associated with electroacupuncture at the Weizhong acupoint (BL 40) promoting muscle repairing ability.Method: A total of 58 male and female Sprague-Dawley rats were divided into a younger group (4-month-old) and an aged group (16-month-old), younger and aged rats were further divided as a sham, injured, injured rats treated with electroacupuncture at Weizhong point or treated with Non-Weizhong point groups. The back muscle injury model was produced in rats as a previously described method with modification. Furthermore, Weizhong acupoints underwent electroacupuncture treatment with 15 V magnitude, 2 Hz/10 Hz frequency density, 1.0 mA current intensity, and 10 min each day for 10 consecutive days using HANS&#039;s electroacupuncture apparatus. After the last treatment, the paravertebral muscles and serum of all animals were undergone histological, immunohistochemistry, and flow cytometry analysis. Serum levels of Creatine Kinase (CK) and proinflammatory cytokine, interleukin 6 (IL-6), were measured separately by using ELISA kit.Results: Electroacupuncture of Weizhong (BL 40) acupoints significantly attenuated back muscle damage in both young and aged rats, increasing PAX7 (a marker of muscle satellite cells) and MYOD (major marker of myoblasts) cells, simultaneously, reducing serum proinflammatory cytokines, IL-6, and downregulation of p38 MAPK signaling in aged muscular satellite cells.Conclusion: Our studies suggest that electroacupuncture of Weizhong (BL 40) acupoints can restore aged back muscular satellite cells and their regeneration capacity. These suggested electroacupuncture may be a potential means of promoting rehabilitation for muscular injury in aged patients. <p><a href="https://greenmedinfo.com/article/electroacupuncture-weizhong-bl-40-acupoints-can-restore-aged-back-muscular-sat" target="_blank">read more</a></p> https://greenmedinfo.com/article/electroacupuncture-weizhong-bl-40-acupoints-can-restore-aged-back-muscular-sat#comments Muscle Damage Anti-Inflammatory Agents Electroacupuncture Interleukin-6 Downregulation Bodily Tissue Regeneration Muscle Regeneration Animal Study Thu, 25 Aug 2022 18:48:54 +0000 greenmedinfo 262427 at https://greenmedinfo.com Lactoferrin as a regenerative agent: the old-new panacea? https://greenmedinfo.com/article/lactoferrin-regenerative-agent-old-new-panacea PMID:  Pharmacol Res. 2021 Mar 17:105564. Epub 2021 Mar 17. PMID: 33744427 Abstract Title:  Lactoferrin as a regenerative agent: the old-new panacea? Abstract:  Lactoferrin (Lf) possesses various biological properties and therapeutic potentials being a perspective anti-inflammatory, antibacterial, antiviral, antioxidant, antitumor, and immunomodulatory agent. A significant body of literature has also demonstrated that Lf modulates regenerative processes in different anatomical structures, such as bone, cartilage, skin, mucosa, cornea, tendon, vasculature, and adipose tissue. Hence, this review collected and analyzed the data on the regenerative effects of Lf, as well as paid specific attention to their molecular basis. Furthermore, tissue and condition-specific activities of different Lf types as well as problems of their delivery to the targeted organs were discussed. The authors strongly hope that this review will stimulate researchers to focus on the highlighted topics thus accelerating the progress of Lf&#039;s wider clinical application. <p><a href="https://greenmedinfo.com/article/lactoferrin-regenerative-agent-old-new-panacea" target="_blank">read more</a></p> https://greenmedinfo.com/article/lactoferrin-regenerative-agent-old-new-panacea#comments Lactoferrin Regenerative Bodily Tissue Regeneration Review Sun, 28 Mar 2021 22:23:56 +0000 greenmedinfo 237104 at https://greenmedinfo.com Lonicerae Japonicae Caulis: a review of its research progress of active metabolites and pharmacological effects. https://greenmedinfo.com/article/lonicerae-japonicae-caulis-review-its-research-progress-active-metabolites-and PMID:  Front Pharmacol. 2023 ;14:1277283. Epub 2023 Oct 25. PMID: 37954842 Abstract Title:  : a review of its research progress of active metabolites and pharmacological effects. Abstract:  is the aboveground stem part of theThunb, which belongs to the medicine food homology species in China. It has the effects of clearing away heat, toxic material, dredging wind and unblocking collaterals. Modern research shows that it contains various active metabolites and a wide range of pharmacological effects, which is of great research and clinical application value. It mainly contains organic acids, volatile oils, flavonoids, triterpenes, triterpene saponins and other active metabolites. Its pharmacological effects mainly include anti-inflammatory, antibacterial, antitumor, antioxidant, and repairing bone and soft tissue. Based on the literature reports in recent years, the active metabolites, pharmacological effects and mechanisms ofwere sorted out and summarized. It lays a foundation for explaining the efficacy material basis and application value of. It aims to provide a reference for the in-depth research, development and utilization of. <p><a href="https://greenmedinfo.com/article/lonicerae-japonicae-caulis-review-its-research-progress-active-metabolites-and" target="_blank">read more</a></p> https://greenmedinfo.com/article/lonicerae-japonicae-caulis-review-its-research-progress-active-metabolites-and#comments Bacterial Infections Honeysuckle Inflammation Oxidative Stress Wound Healing Anti-Bacterial Agents Anti-Inflammatory Agents Antioxidants Bodily Tissue Regeneration Review Wed, 06 Dec 2023 00:32:06 +0000 greenmedinfo 284302 at https://greenmedinfo.com Mesenchymal Stem Cells Synergize with 635, 532, and 405 nm Laser Wavelengths in Renal Fibrosis. https://greenmedinfo.com/article/mesenchymal-stem-cells-synergize-635-532-and-405-nm-laser-wavelengths-renal-fi PMID:  Photomed Laser Surg. 2016 Nov ;34(11):556-563. Epub 2016 May 31. PMID: 27244220 Abstract Title:  Mesenchymal Stem Cells Synergize with 635, 532, and 405 nm Laser Wavelengths in Renal Fibrosis: A Pilot Study. Abstract:  OBJECTIVE: To address whether a single treatment of one of three visible light wavelengths, 635, 532, and 405 nm (constant wave, energy density 2.9 J/m), could affect the hallmarks of established renal fibrosis and whether these wavelengths could facilitate mesenchymal stem cell (MSC) beneficence.BACKGROUND DATA: Chronic kidney disease is a global health problem with only 20% receiving care worldwide. Kidneys with compromised function have ongoing inflammation, including increased oxidative stress and apoptosis, peritubular capillary loss, tubular atrophy, and tubulointerstitial fibrosis. Promising studies have highlighted the significant potential of MSC-based strategies to mitigate fibrosis; however, reversal of established fibrosis has been problematic, suggesting that methods to potentiate MSC effects require further development. Laser treatments at visible wavelengths have been reported to enhance mitochondrial potential and available cellular ATP, facilitate proliferation, and inhibit apoptosis. We hypothesized that laser-delivered energy might provide wavelength-specific effects in the fibrotic kidney and enhance MSC responses.MATERIALS AND METHODS: Renal fibrosis, established in C57BL6 mice following 21 days of unilateral ureter obstruction (UUO), was treated with one of three wavelengths alone or with autologous MSC. Mitochondrial activity, cell proliferation, apoptosis, and cytokines were measured 24 h later.RESULTS: Wavelengths 405, 532, and 635 nm all significantly synergized with MSC to enhance mitochondrial activity and reduce apoptosis. Proliferative activity was observed in the renal cortices following combined treatment with the 532 nm laser and MSC; endothelial proliferation increased in response to the 635 nm laser alone andto the combined effects of MSC and the 405 nm wavelength. Reductions of transforming growth factor-β were observed with 532 nm alone and when combined with MSC.CONCLUSIONS: Specific wavelengths of laser energy appear to induce different responses in renal fibrotic tissue. These findings support further study in the development of a customized laser therapy program of combined wavelengths to optimize MSC effects in the treatment of renal fibrosis. <p><a href="https://greenmedinfo.com/article/mesenchymal-stem-cells-synergize-635-532-and-405-nm-laser-wavelengths-renal-fi" target="_blank">read more</a></p> https://greenmedinfo.com/article/mesenchymal-stem-cells-synergize-635-532-and-405-nm-laser-wavelengths-renal-fi#comments Renal Fibrosis Anti-Fibrotic Laser Treatment Bodily Tissue Regeneration Animal Study Fri, 21 Feb 2020 20:37:41 +0000 greenmedinfo 212938 at https://greenmedinfo.com Molecular targets of pinocembrin underlying its regenerative activities in human keratinocytes. https://greenmedinfo.com/article/molecular-targets-pinocembrin-underlying-its-regenerative-activities-human-ker PMID:  Pharmaceuticals (Basel). 2022 Jul 31 ;15(8). Epub 2022 Jul 31. PMID: 36015102 Abstract Title:  Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human Keratinocytes. Abstract:  Pinocembrin is one of the well-known compounds in the group of flavonoids. The pharmacological activities of pinocembrin in association with wound-healing activities have been reported. However, its effects on the aspect of cellular interaction underlying growth and survival are still unidentified in human keratinocytes. Our previous study reported thatpotently stimulated survival and proliferation of a human keratinocyte cell line (HaCaT). On the basis that pinocembrin is revealed to be one of the major constituents of this plant, we aimed to define the survival- and proliferation-enhancing effects of this compound at the cellular level. Results from the current study confirmed that pinocembrin induced an increase in HaCaT cell number. At the signaling perspective, we identified that pinocembrin significantly triggered ERK1/2 and Akt activation. The stimulating effects of pinocembrin were clearly inhibited by MEK and PI3K inhibitors authenticating that proliferation- and survival-promoting activities of pinocembrin were mainly acted on these two signaling cascades. Altogether, we successfully identified that pinocembrin functions to induce keratinocyte proliferation and survival, at least by provoking MAPK and PI3K pathways. Our study encourages the fact that pinocembrin is one of the interesting natural flavonoid compounds to be developed as a wound closure-promoting agent. <p><a href="https://greenmedinfo.com/article/molecular-targets-pinocembrin-underlying-its-regenerative-activities-human-ker" target="_blank">read more</a></p> https://greenmedinfo.com/article/molecular-targets-pinocembrin-underlying-its-regenerative-activities-human-ker#comments Flavonoids Wound Healing Bodily Tissue Regeneration In Vitro Study Fri, 26 Aug 2022 22:35:07 +0000 greenmedinfo 262477 at https://greenmedinfo.com Nigella sativa and thymoquinone have been shown to promote wound healing, attenuate tissue inflammation, and prevent organ fibrosis via regulation of the epithelial to mesenchymal transition process. https://greenmedinfo.com/article/nigella-sativa-and-thymoquinone-have-been-shown-promote-wound-healing-attenuat PMID:  BMC Complement Altern Med. 2019 Oct 30 ;19(1):290. Epub 2019 Oct 30. PMID: 31666058 Abstract Title:  Effect of Nigella sativa and its bioactive compound on type 2 epithelial to mesenchymal transition: a systematic review. Abstract:  BACKGROUND: Nigella sativa or commonly known as black seed or black cumin is one of the most ubiquitous complementary medicine. Epithelial to mesenchymal transition (EMT) of type 2 is defined by the balance between wound healing and tissue fibrosis, which is dependent to the state of inflammation. This systematic review is conducted to provide an overview regarding the reported effect of Nigella sativa and its bioactive compound on the type 2 EMT.METHODS: A search was done in EBSCOHOST, OVID and SCOPUS database to obtain potentially relevant articles that were published between 1823 and August 2019. This review includes studies that focus on the effect of Nigella sativa and its bioactive compound on the events related to type 2 EMT.RESULTS: A total of 1393 research articles were found to be potentially related to the effect of Nigella sativa and its bioactive compound, thymoquinone on Type 2 EMT. After screening was done, 22 research articles met inclusion criteria and were included in this review. Majority of the studies, reported better wound healing rate or significant prevention of tissue inflammation and organ fibrosis following Nigella sativa or thymoquinone treatments. In terms of wound healing, studies included reported progression of EMT related pathological changes after treatment with Nigella sativa or thymoquinone. Alternatively, in terms of fibrosis and inflammation, studies included reported reversal of pathological changes related to EMT after treatment with Nigella sativa or thymoquinone.CONCLUSION: Through this review, Nigella sativa and thymoquinone have been associated with events in Type 2 EMT. They have been shown to promote wound healing, attenuate tissue inflammation, and prevent organ fibrosis via regulation of the EMT process. <p><a href="https://greenmedinfo.com/article/nigella-sativa-and-thymoquinone-have-been-shown-promote-wound-healing-attenuat" target="_blank">read more</a></p> https://greenmedinfo.com/article/nigella-sativa-and-thymoquinone-have-been-shown-promote-wound-healing-attenuat#comments Fibrosis Nigella sativa (aka Black Seed) Thymoquinone Anti-Fibrotic Bodily Tissue Regeneration Phytotherapy Plant Extracts Review Fri, 21 Feb 2020 20:29:38 +0000 greenmedinfo 212931 at https://greenmedinfo.com Protective effect of grape and apple juices against cadmium intoxication in the kidney. https://greenmedinfo.com/article/protective-effect-grape-and-apple-juices-against-cadmium-intoxication-kidney PMID:  Drug Res (Stuttg). 2020 Aug 20. Epub 2020 Aug 20. PMID: 32820471 Abstract Title:  Protective Effect of Grape and Apple Juices against Cadmium Intoxication in the Kidney of Rats. Abstract:  BACKGROUND: The objective of this study was to evaluate protective effect of grape and apple juices against toxicity induced by cadmium in the kidney of rats.METHODS: A total of 20 male-Wistar rats were distributed into four groups (n=5): Control group: animals received an intraperitoneal (i.p.) injection of 0.9% saline solution and after 15 days, 1 mL of water was administered for 15 days, via gavage; Cadmium group: animals received an intraperitoneal injection of cadmium chloride (1.2 mg/kg) and after 15 days, 1 mL of water was administered for 15 days via gavage; Cadmium+Grape Juice: animals received an i.p. injection of cadmium chloride (1.2 mg/kg), and after 15 days, 0.8 mL of grape juice was administered for 15 days, via gavage; Cadmium+Apple Juice: animals received i.p. injection of cadmium chloride (1.2 mg/kg) and after 15 days, 1.0 mL of apple juice was administered for 15 days, via gavage.RESULTS: Histopathological analysis revealed severe tubular lesion and necrosis in the group exposed to cadmium, while animals exposed to grape or apple juices showed a significant reduction of tissue injury. 8-OHdG immunoexpression, DNA damage, cytochrome C and catalase gene expressions and Toll like signaling pathway (TLR2, and pIKKα/β) decreased in animals treated with grape juice when compared to cadmium group.CONCLUSION: Taken together, we conclude that grape and apple juices had a protective effect by means of antioxidant, antigenotoxic actions and for promoting tissue regeneration in the kidney of rats following cadmium intoxication. <p><a href="https://greenmedinfo.com/article/protective-effect-grape-and-apple-juices-against-cadmium-intoxication-kidney" target="_blank">read more</a></p> https://greenmedinfo.com/article/protective-effect-grape-and-apple-juices-against-cadmium-intoxication-kidney#comments Apple Cadmium Poisoning Grape Kidney Damage: Chemically-Induced Cadmium Genoprotective Regenerative Renoprotective Bodily Tissue Regeneration Animal Study Sat, 05 Sep 2020 17:03:25 +0000 greenmedinfo 226364 at https://greenmedinfo.com Purified astaxanthin from Haematococcus pluvialis promotes tissue regeneration. https://greenmedinfo.com/article/purified-astaxanthin-haematococcus-pluvialis-promotes-tissue-regeneration PMID:  Oxid Med Cell Longev. 2020 ;2020:4946902. Epub 2020 Aug 3. PMID: 32832000 Abstract Title:  Purified Astaxanthin fromPromotes Tissue Regeneration by Reducing Oxidative Stress and the Secretion of Collagenand. Abstract:  Intracellular reactive apoptosis and reactive oxygen species (ROS) play a crucial role in ultraviolet- (UV-) induced inflammation and aging reaction in human dermal tissues. This study determines the mechanism by whichextracts (HPE) and purified astaxanthin (HPA) to promote skin regeneration in the injured tissueand. The results show that HPE and HPA decrease the DNA damage and promote the secretion of collagen from the human normal fibroblast cell line (Hs68) in a dose-dependent manner. UV irradiation and HPA reduce oxidative stress damage due to phorbol-12-myristate-13-acetate (PMA). When skin cells are injured by free radicals, cells undergo a programmed cellular death. Cellular apoptotic death is determined using annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining to verify that there is no cell membrane asymmetry and that the nuclear membrane is broken. Inflammatory symptoms and apoptotic injuries to experimental rats in a group that is treated with HPA treated are decreased in a dose-dependent manner after UVB exposure (300 mJ/cm) for 15 min, compared to the vehicle control group. These positive results show that HPA repairs UVB-triggered skin tissue injury and aging by conducting electrons out of cells to maintain a low level of oxidative stress so that collagen is synthesizedand. <p><a href="https://greenmedinfo.com/article/purified-astaxanthin-haematococcus-pluvialis-promotes-tissue-regeneration" target="_blank">read more</a></p> https://greenmedinfo.com/article/purified-astaxanthin-haematococcus-pluvialis-promotes-tissue-regeneration#comments Astaxanthin Ultraviolet Radiation Induced Damage Antioxidants Regenerative Bodily Tissue Regeneration Animal Study In Vitro Study Fri, 28 Aug 2020 17:45:38 +0000 greenmedinfo 225888 at https://greenmedinfo.com Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates. https://greenmedinfo.com/article/resveratrol-enhances-functionality-and-improves-regeneration-mesenchymal-stem- PMID:  Exp Mol Med. 2018 06 27 ;50(6):1-15. Epub 2018 Jun 27. PMID: 29959311 Abstract Title:  Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates. Abstract:  Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients&#039; own MSCs) in regenerative medicine has developed considerable interest. However, the impaired cell viability and regenerative potential following MSCs impacted by disease remain a major challenge. Resveratrol (RSV) exhibits reliable and extensive rejuvenative activities that have received increasing clinical attention. Here, we uncovered that resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates. Periodontal ligament MSCs (PDLSCs) from normal control subjects (N-PDLSCs) and periodontitis patients (P-PDLSCs) were investigated. Compared to N-PDLSCs, P-PDLSCs were less capable of forming cell aggregates, and P-PDLSC aggregates showed impaired osteogenesis and regeneration. These functional declines could be mimicked in N-PDLSCs by tumor necrosis factor alpha (TNF-α) treatment. Notably, a TNF-α-induced functional decline in N-PDLSC aggregates was rescued by RSV application. More importantly, in both N-PDLSCs and P-PDLSCs, RSV promoted cell aggregate formation and improved their osteogenic potential. Furthermore, as proven ectopically in vivo, the tissue regenerative capability of P-PDLSC aggregates was also enhanced after RSV treatment during aggregate formation in vitro. Finally, in a rat in situ regeneration model, we successfully applied both N-PDLSC aggregates and P-PDLSC aggregates to repair periodontal defects upon long-term functional improvements by RSV preconditioning. Together, our data unravel a novel methodology for using pharmacology (i.e., RSV)-based cell aggregate engineering to improve the functionality and facilitate the regeneration of MSCs from both healthy and inflammatory microenvironments, shedding light on improving the application of autologous MSC-mediated regenerative medicine. <p><a href="https://greenmedinfo.com/article/resveratrol-enhances-functionality-and-improves-regeneration-mesenchymal-stem-" target="_blank">read more</a></p> https://greenmedinfo.com/article/resveratrol-enhances-functionality-and-improves-regeneration-mesenchymal-stem-#comments Periodontitis Resveratrol Regenerative Bodily Tissue Regeneration In Vitro Study Sun, 16 Oct 2022 00:40:38 +0000 greenmedinfo 264982 at https://greenmedinfo.com Systemic administration of curcumin or piperine enhances the periodontal repair. https://greenmedinfo.com/article/systemic-administration-curcumin-or-piperine-enhances-periodontal-repair PMID:  Clin Oral Investig. 2018 Nov 29. Epub 2018 Nov 29. PMID: 30498979 Abstract Title:  Systemic administration of curcumin or piperine enhances the periodontal repair: a preliminary study in rats. Abstract:  OBJECTIVES: Studies have documented the anti-inflammatory effects of spices, which may be related to treatment of chronic diseases. The purpose of this study was to evaluate the influence of curcumin and piperine and their association on experimental periodontal repair in rats.MATERIALS AND METHODS: Periodontitis was induced via the installation of a ligature around the first molar. After 15 days, the ligatures were removed, and the rats were separated into groups (12 animals per group): (i) curcumin, (ii) piperine, (iii) curcumin+piperine, (iv) corn oil vehicle, and (v) control group (animals had ligature-induced periodontitis but were not treated). The compounds were administered daily, for 15 days by oral gavage. Animals were euthanized at 5 and 15 days, and hemimaxillae and gingival tissues were harvested. Bone repair was assessed by μCT (microcomputer tomography). Histological sections were stained with hematoxylin/eosin (H/E) for the assessment of cellular infiltrate orpicrosirius red for quantification of collagen content, and subjected to immunohistochemistry for detecting NF-ĸB. Gingival tissues were used to evaluate levels of TGF-β and IL-10 (ELISA).RESULTS: Curcumin and piperine increased the TGF-β level, significantly improved the collagen repair, and decreased the cellularity and activation of NF-ĸB in the periodontal tissues, but only curcumin caused a significant increase in early bone repair.CONCLUSION: Curcumin and piperine promoted a substantive effect on tissue repair; however, there was not synergistic effect of compounds administered in combination.CLINICAL RELEVANCE: Curcumin and piperine stimulates the tissue repair and may be potential candidates for the treatment of periodontal disease. <p><a href="https://greenmedinfo.com/article/systemic-administration-curcumin-or-piperine-enhances-periodontal-repair" target="_blank">read more</a></p> https://greenmedinfo.com/article/systemic-administration-curcumin-or-piperine-enhances-periodontal-repair#comments Curcumin Periodontitis Piperine Bodily Tissue Regeneration Bone Regeneration Animal Study Sat, 02 Mar 2019 06:59:22 +0000 greenmedinfo 180626 at https://greenmedinfo.com Tanshinone IIA is a viable therapeutic option for regenerative medical approaches aimed at the treatment of periodontitis. https://greenmedinfo.com/article/tanshinone-iia-viable-therapeutic-option-regenerative-medical-approaches-aimed PMID:  Am J Transl Res. 2019 ;11(1):340-350. Epub 2019 Jan 15. PMID: 30787991 Abstract Title:  Tanshinone IIA promotes osteogenic differentiation of human periodontal ligament stem cells via ERK1/2-dependent Runx2 induction. Abstract:  Mesenchymal stem cells (MSCs) of the dental or craniofacial origin include Human periodontal ligament stem cells (hPDLSCs), which are able to readily differentiate into osteoblasts. Tanshinone IIA (TSA) is a diterpene quinone compound that is derived from Danshen (also known as Salvia miltiorrhiza) used frequently in the context of traditional Chinese medicine (TCM). This study sought to assess how TSA affects the osteogenic differentiation of hPDLSCs. We found that TSA promotes both this differentiation and hPDLSC maturation. This was dependent on TSA-mediated activation of the ERK1/2 signaling pathway, and ERK1/2 inhibition disrupted TSA-induced Runx2 expression. From these results, we conclude that TSA can induce hPDLSC osteogenesis through the ERK1/2-Runx2 axis, suggesting that TSA is a viable therapeutic option for regenerative medical approaches aimed at the treatment of periodontitis. <p><a href="https://greenmedinfo.com/article/tanshinone-iia-viable-therapeutic-option-regenerative-medical-approaches-aimed" target="_blank">read more</a></p> https://greenmedinfo.com/article/tanshinone-iia-viable-therapeutic-option-regenerative-medical-approaches-aimed#comments Periodontitis Tanshinone IIA Regenerative Bodily Tissue Regeneration Human In Vitro Mon, 15 Apr 2019 11:49:44 +0000 greenmedinfo 185032 at https://greenmedinfo.com Tetramethylpyrazine/ligustrazine, curcumin, and polyglucoside of Tripterygium have experimentally shown good potential for improving renal interstitial fibrosis. https://greenmedinfo.com/article/tetramethylpyrazineligustrazine-curcumin-and-polyglucoside-tripterygium-have-e PMID:  J Integr Med. 2016 May ;14(3):165-73. PMID: 27181123 Abstract Title:  Interventional mechanisms of herbs or herbal extracts on renal interstitial fibrosis. Abstract:  Renal interstitial fibrosis (RIF) is a common development in chronic renal diseases that can lead to uremia and be life-threatening. The RIF pathology has complicated extracellular and intercellular mechanisms, involving many cells and cytokines, resulting in an incomplete mechanistic understanding of the disease. Finding effective herbs or herbal extracts for prevention and treatment of RIF is crucial because current medical approaches do not reliably slow or reverse RIF. In recent years, many experts have worked to identify herbs or herbal extracts to combat RIF both in vivo and in vitro, with some success. This review attempts to summarize the possible interventional mechanisms of herbs or herbal extracts involved in protecting and reversing RIF. The authors found some herbs and their extracts that may ameliorate renal impairments through anti-inflammation, anti-fibrogenesis and stabilization of extra cellular matrix. Among them, tetramethylpyrazine/ligustrazine, curcumin and polyglucoside of Tripterygium have experimentally shown good potential for improving RIF. However, conclusive evidence is still needed, especially in randomized controlled clinical trials. We expect that herbs or herbal extracts will play an important role in RIF treatment and reversal in the future. <p><a href="https://greenmedinfo.com/article/tetramethylpyrazineligustrazine-curcumin-and-polyglucoside-tripterygium-have-e" target="_blank">read more</a></p> https://greenmedinfo.com/article/tetramethylpyrazineligustrazine-curcumin-and-polyglucoside-tripterygium-have-e#comments Renal Fibrosis Bodily Tissue Regeneration Phytotherapy Plant Extracts Review Fri, 21 Feb 2020 20:35:13 +0000 greenmedinfo 212935 at https://greenmedinfo.com Vitamin C enema advances induction of remission in the dextran sodium sulfate-induced colitis model. https://greenmedinfo.com/article/vitamin-c-enema-advances-induction-remission-dextran-sodium-sulfate-induced-co PMID:  J Nutr Sci Vitaminol (Tokyo). 2021 ;67(2):91-98. PMID: 33952740 Abstract Title:  Vitamin C Enema Advances Induction of Remission in the Dextran Sodium Sulfate-Induced Colitis Model in Rats. Abstract:  The current main treatment for ulcerative colitis (UC) is induction therapy by long-term administration of 5-aminosalicylic acid (5-ASA), but various side effects have been reported. Therefore, a radical cure for UC is desired. A vitamin C (VC) has anti-inflammatory effects. Therefore, this study investigated whether a VC solution enema shortens induction of remission in colitis model rats. Wistar rats (6 wk old/male) were allowed to freely ingest a 1% dextran sulfate sodium (DSS) solution for 10 d and then switched to tap water for normal breeding for 10 d (UC group). At the time of switching to tap water, an enema was performed with a 5-ASA solution (40 mg/kg/d) or VC solution (460 mg/kg/d) for 10 d. The neutrophil number, COX-2, which is an index of inflammation, and type III collagen, which is an early healing marker, were significantly increased in the UC group. However, the VC group showed decreases compared with UC groups. Furthermore, compared with UC and 5-ASA groups, the VC group showed increased expression of type I collagen, which is expressed late in healing, and significant epithelial regeneration was observed in colon tissue. The VC solution enema shortened the induction of remission by directly suppressing inflammation of damaged large intestinal tissues and promoting mucosal healing. <p><a href="https://greenmedinfo.com/article/vitamin-c-enema-advances-induction-remission-dextran-sodium-sulfate-induced-co" target="_blank">read more</a></p> https://greenmedinfo.com/article/vitamin-c-enema-advances-induction-remission-dextran-sodium-sulfate-induced-co#comments Colitis Vitamin C Anti-Inflammatory Agents Gastroprotective Bodily Tissue Regeneration Animal Study Fri, 07 May 2021 23:24:54 +0000 greenmedinfo 239271 at https://greenmedinfo.com